A PREALBUMIN ACTIVATOR OF PREKALLIKREIN
نویسندگان
چکیده
منابع مشابه
A Prealbumin Activator of Prekallikrein
Activation of a plasma fraction containing unactivated Hageman factor and prekallikrein followed by chromatography of this fraction on DEAE-cellulose revealed four peaks having bradykinin-generating activity. Peak 1 contained kallikrein; peaks 2-3, 4, and 5 each contained prekallikrein-activating activity. Elution of peaks 2-3, 4, and 5 from disc gels after electrophoresis at pH 9.3 revealed pe...
متن کاملA PREALBUMIN ACTIVATOR OF PREKALLIKREIN II. DERIVATION OF ACTIVATORS OF PREKALLIKREIN FROM ACTIVE HAGEMAN FACTOR BY DIGESTION WITtt
Bradykinin is generated in human plasma by the action of plasma kallikrein upon kininogen (1, 2). Although Hageman factor is required for the formation of kallikrein (3, 4), the role of Hageman factor in the formation of the prekallikrein activator is not clearly delineated. Kaplan and Austen (5) have described a prekallikrein activator present in human serum which has a prealbumin mobility on ...
متن کاملOrigin of the high molecular weight activator of prekallikrein.
Two lines of evidence suggest that the high molecular weight activator of prekallikrein (large activator) (BAGDASARIAN, A., TALAMO, R. C., AND COLMAN, R. W. (1973) J. Biol. Chem. 248, 3456-3463) is derived from coagulation factor XII (Hageman factor). Large activator could not be isolated from plasma congenitally deficient in factor XII, and partial immunologic identity was demonstrated between...
متن کاملIdentification of prolylcarboxypeptidase as the cell matrix-associated prekallikrein activator.
Investigations determined that the cell matrix-associated prekallikrein (PK) activator is prolylcarboxypeptidase. PK activation on human umbilical vein endothelial cell (HUVEC) matrix is inhibited by antipain (IC(50)=50 microM) but not anti-factor XIIa antibody, 3 mM benzamidine, 5 mM iodoacetic acid or iodoacetamide, or 3 mM N-ethylmaleimide. Corn trypsin inhibitor (IC(50)=100 nM) or Fmoc-amin...
متن کاملAnaphylactic release of a prekallikrein activator from human lung in vitro.
We have demonstrated the in vitro IgE-mediated release of a prekallikrein activator from human lung. The lung prekallikrein activator was partially purified by sequential chromatography on sulfopropyl-Sephadex, DEAE-Sephacel, and Sepharose 6B. Purified human prekallikrein was converted to its active form (kallikrein) by the lung protease. The generated kallikrein was shown to be biologically ac...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1971
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.133.4.696